# PhiC31integrase Mediated Transgenesis

This is a preferred method of transgenesis as this is site directed and hence positional effects are minimized.
Site-Specific Excel - Injection of DNA into the embryos of PhiC31 integrase & selected attP docking site (click here and choose attP sites), crossing of G0 flies, screening the transformants and balancing the insertions.
Site-Specific BAC - This service includes injecting the BACs or fosmids (>30kb) into 500 embryos of strain (or a cross of) PhiC31-integrase and a docking site of the user’s choice and scoring the transformants, balancing and shipping.
Site-Specific CRISPR - This service includes injecting the plasmids with CRISPR/Cas9 guide RNA into 300 embryos of strain (or a cross of) PhiC31-integrase and a docking site of the user’s choice and scoring the transformants, balancing and shipping.
To avail the services, kindly register at http://ccamp.res.in/FlyFacility/user and fill in the requisite form at http://ccamp.res.in/FlyFacility/newrequest
User guidelines can be found here ( https://www.ncbs.res.in/research-facilities/drosophila-userguidelines )
The following fly stocks are used for the site directed transgenesis.

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